LOCUS       pSTV2_C-BirA_pDe        9280 bp    DNA     circular     30-AUG-2018
DEFINITION  .
ACCESSION   urn.local...7v-9fz0ltg
VERSION
KEYWORDS    .
SOURCE      null
  ORGANISM  
            .
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com Cat.
            No. 632158,632162,632157,632163.
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label="5'\LTR"
     misc_binding    636..653
                     /label="PBS"
     misc_feature    685..822
                     /label="Packaging\signal"
     misc_binding    1303..1536
                     /label="RRE"
     misc_feature    2028..2151
                     /label="cPPT/CTS"
     promoter        2205..2520
                     /modified_by="User"
                     /label="TREtight"
     attR1           2573..2697
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="ATTR1"
     CDS             2806..3465
                     /ApEinfo_fwdcolor="#00ff28"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Chlorm"
     CDS             3807..4112
                     /ApEinfo_fwdcolor="#00ff28"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="ccdb"
     attR2           4153..4277
                     /created_by="User"
                     /modified_by="User"
                     /label="ATTR2"
     CDS             4288..5250
                     /ApEinfo_fwdcolor="#006cff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BirA"
     CDS             5281..5307
                     /ApEinfo_fwdcolor="#006cff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Flag"
     WPRE            5324..5899
                     /ApEinfo_fwdcolor="#008cff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="WPRE"
     LTR             5996..6632
                     /label="3'\LTR"
     rep_origin      complement(7101..7774)
                     /label="pUC\origin"
     CDS             complement(7919..8915)
                     /label="Amp(R)"
ORIGIN      
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
     2281 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2341 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
     2401 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
     2461 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2521 tggagaagga tccgcggccg cgccggctct agatcgcgaa cgcgagcttt agacaagttt
     2581 gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat
     2641 tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt cactatggcg
     2701 gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt
     2761 ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     2821 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     2881 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     2941 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     3001 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     3061 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     3121 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     3181 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     3241 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     3301 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     3361 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     3421 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaagatc tggatccggc
     3481 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
     3541 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
     3601 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
     3661 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
     3721 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
     3781 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
     3841 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
     3901 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
     3961 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
     4021 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
     4081 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
     4141 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
     4201 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
     4261 ttcttgtaca aagtggttga ttctagaatg aaggacaaca ccgtgcccct gaagctgatc
     4321 gccctgctgg ccaacggcga gttccacagc ggcgagcagc tgggcgagac cctgggcatg
     4381 agccgggccg ccatcaacaa gcacatccag accctgcggg actggggcgt ggacgtgttc
     4441 accgtgcccg gcaagggcta cagcctgccc gagcccatcc agctgctgaa cgccaagcag
     4501 atcctgggcc agctggacgg cggcagcgtg gccgtgctgc ccgtgatcga cagcaccaac
     4561 cagtacctgc tggaccggat cggcgagctg aagagcggcg acgcctgcat cgccgagtac
     4621 cagcaggccg gccggggcgg ccggggccgg aagtggttca gccccttcgg cgccaacctg
     4681 tacctgagca tgttctggcg gctggagcag ggccccgccg ccgccatcgg cctgagcctg
     4741 gtgatcggca tcgtgatggc cgaggtgctg cggaagctgg gcgccgacaa ggtgcgggtg
     4801 aagtggccca acgacctgta cctgcaggac cggaagctgg ccggcatcct ggtggagctg
     4861 accggcaaga ccggcgacgc cgcccagatc gtgatcggcg ccggcatcaa catggccatg
     4921 cggcgggtgg aggagagcgt ggtgaaccag ggctggatca ccctgcagga ggccggcatc
     4981 aacctggacc ggaacaccct ggccgccatg ctgatccggg agctgcgggc cgccctggag
     5041 ctgttcgagc aggagggcct ggccccctac ctgagccggt gggagaagct ggacaacttc
     5101 atcaaccggc ccgtgaagct gatcatcggc gacaaggaga tcttcggcat cagccggggc
     5161 atcgacaagc agggcgccct gctgctggag caggacggca tcatcaagcc ctggatgggc
     5221 ggcgagatca gcctgcggag cgccgagaag gggggatctg gccccggcgg cggcgcgcca
     5281 gactacaaag acgatgacga taaataactc gagcagtcga caatcaacct ctggattaca
     5341 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     5401 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     5461 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5521 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5581 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5641 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5701 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     5761 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5821 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5881 gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac ctttaagacc
     5941 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga
     6001 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
     6061 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     6121 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     6181 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     6241 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     6301 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     6361 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     6421 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     6481 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6541 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6601 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     6661 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt
     6721 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6781 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6841 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6901 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6961 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7021 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7081 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7141 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7201 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7261 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7321 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7381 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7441 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7501 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7561 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7621 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7681 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7741 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7801 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7861 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7921 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7981 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8041 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8101 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8161 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8221 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8281 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8341 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8401 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8461 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8521 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8581 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8641 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8701 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8761 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8821 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8881 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt
     8941 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     9001 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     9061 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
     9121 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
     9181 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa
     9241 gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
